Translational_Unit

Part:BBa_I716337:Design

Designed by: nhu nguyen   Group: iGEM07_Berkeley_UC   (2007-08-14)


rbs-ThpC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 32
    Illegal PstI site found at 475
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 32
    Illegal PstI site found at 475
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal prefix found in sequence at 32
    Illegal suffix found in sequence at 460
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 32
    Illegal PstI site found at 475
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 32
    Illegal PstI site found at 475
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 181
    Illegal BsaI.rc site found at 205
    Illegal BsaI.rc site found at 280
    Illegal BsaI.rc site found at 1083


Design Notes

Digest pBca9145-BBa I716333 (BglII/XhoI, 2063bp, 405bp, S) Sub into pJD507-pSB1A2C (BamHI/XhoI, 2071bp, 9bp, L) Product is I716337


Source


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

References